NaOH low-salt method for chloroplast isolation and highly pure cpDNA preparation from Aeluropus littoralis
نویسندگان
چکیده
منابع مشابه
Isolation, molecular cloning and expression analysis of Aeluropus littoralis Monodehydroascorbate reductase (MDHAR) gene under salt stress
Plants are naturally exposed to frequent changes in environmental conditions such as salt stress affecting their growth and development, and consequently a considerable reduction in total productivity. Monodehydroascorbate reductase (MDHAR), a key enzyme in ascorbate-glutathione cycle, serves as an important antioxidative enzyme in scavenging of reactive oxygen spices (ROS). In this study, ...
متن کاملisolation, molecular cloning and expression analysis of aeluropus littoralis monodehydroascorbate reductase (mdhar) gene under salt stress
plants are naturally exposed to frequent changes in environmental conditions such as salt stress affecting their growth and development, and consequently a considerable reduction in total productivity. monodehydroascorbate reductase (mdhar), a key enzyme in ascorbate-glutathione cycle, serves as an important antioxidative enzyme in scavenging of reactive oxygen spices (ros). in this study, mdha...
متن کاملComparative expression profiling of four salt-inducible genes from Aeluropus littoralis
Abiotic stresses such as salinity influence agricultural production. Plants generally respond to stimulus conditions in a complex manner involving many genes and proteins. In the evolution process, halophyte plant Aeluropus littoralis has been proven to have abiotic stress-tolerance capacity. A. littoralis is a salt-resistant halophyte providing a wealthy genetic resource for developing salinit...
متن کاملExpression analysis of salt stress related expressed sequence tags (ESTs) from Aeluropus littoralis by quantitative real-time PCR
Aeluropus littoralis is a monocot halophyte grass and provide valuable genetic resources for understanding the molecular mechanisms of stress-responsive genes, and improving tolerance to abiotic stresses in economically important crops. In an attempt to identify salt stressed responsive genes, 154 isolated expressed sequence tags (EST) from A. littoralis were bioinformatically analyzed and func...
متن کاملIncorporation of Na+/H+ antiporter gene from Aeluropus littoralis confers salt tolerance in soybean (Glycine max L..
To develop a salt-tolerant soybean (Glycine max L.) cultivar, a minimal linear Na+/H+ antiporter gene cassette (35S CaMV promoter, open-reading-frame of AINHX1 from Aeluropus littoralis and NOS terminator) was successfully expressed in soybean cultivar TF-29. Southern and Northern blot analysis showed that AlNHX1 was successfully incorporated into the genome and expressed in the transgenic plan...
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ژورنال
عنوان ژورنال: Applications in Plant Sciences
سال: 2018
ISSN: 2168-0450
DOI: 10.1002/aps3.1183